Diploid sugar beet was grown in nutrient culture with ammonium and nitrate N in solutions kept at constant pH by automatic titration. Since plants given nitrate N yielded only 12% more than those given ammonium N, the effects of form of N on plant chemical composition could be determined. The excess of cations in the plant (C-A) was >3 times as great in plants given nitrate as in those given ammonium. The acidity evolved into the culture medium was a good measure of ammonium uptake, whereas with nitrate the amount of alkalinity evolved was unrelated to and small in comparison with the amount of nitrate absorbed. The rate of H+ production decreased during darkness and that of OH- decreased during or just after the dark phase. Levels of solub...