There is an increased interest in plasmid DNA as therapeutics. This is evident in the number of ongoing clinical trials involving the use of plasmid DNA. In order to be an effective therapeutic, high yield and high level of supercoiling are required. From the bioprocessing point of view, the supercoiling level potentially has an impact on the ease of downstream processing. We approached meeting these requirements through plasmid engineering. A 7.2 kb plasmid was developed by the insertion of a bacteriophage Mu strong gyrase-binding sequence (Mu-SGS) to a 6.8 kb pSVβ-Gal and it was used to transform four different E. coli strains, and cultured in order to investigate the Mu-SGS effect and dependence on strain. There was an increase of over 2...
Plasmids DNA (pDNA) are small, circular pieces of DNA used to deliver genetic information and are ke...
Renewed interest in plasmid DNA (pDNA) production is the result of its role in the supply chain of n...
Advances in recombinant DNA technology are leading to a novel generation of medicines for the treatm...
There has been a rise in the interest of plasmid DNA as therapeutics. This is evident in the number ...
There is an increased interest in plasmid DNA as therapeutics. This is evident in the number of ongo...
This study was set out to develop an approach for producing highly supercoiled plasmid DNA. Potentia...
With the recent revival of the promise of plasmid DNA vectors in gene therapy, a novel synthetic bio...
There has been a rise in the interest of plasmid DNA as therapeutics. The rise is evident in the num...
The demand for plasmid DNA (pDNA) is anticipated to increase significantly as DNA vaccines and non-v...
Plasmid DNA (pDNA) has become very attractive as a biopharmaceutical, especially for gene therapy an...
Voß C, Schmidt T, Schleef M, Friehs K, Flaschel E. Production of supercoiled multimeric plasmid DNA ...
Plasmid-based vaccines and therapeutics have been making their way into the clinic in the last years...
To commercialize DNA medicines, industrial plasmid DNA manufacturing processes are needed which meet...
Supercoiled state corresponds to the active form for plasmid applications. The relaxed circular for...
The interest in plasmid DNA (pDNA) as a biopharmaceutical has been increasing over the last several ...
Plasmids DNA (pDNA) are small, circular pieces of DNA used to deliver genetic information and are ke...
Renewed interest in plasmid DNA (pDNA) production is the result of its role in the supply chain of n...
Advances in recombinant DNA technology are leading to a novel generation of medicines for the treatm...
There has been a rise in the interest of plasmid DNA as therapeutics. This is evident in the number ...
There is an increased interest in plasmid DNA as therapeutics. This is evident in the number of ongo...
This study was set out to develop an approach for producing highly supercoiled plasmid DNA. Potentia...
With the recent revival of the promise of plasmid DNA vectors in gene therapy, a novel synthetic bio...
There has been a rise in the interest of plasmid DNA as therapeutics. The rise is evident in the num...
The demand for plasmid DNA (pDNA) is anticipated to increase significantly as DNA vaccines and non-v...
Plasmid DNA (pDNA) has become very attractive as a biopharmaceutical, especially for gene therapy an...
Voß C, Schmidt T, Schleef M, Friehs K, Flaschel E. Production of supercoiled multimeric plasmid DNA ...
Plasmid-based vaccines and therapeutics have been making their way into the clinic in the last years...
To commercialize DNA medicines, industrial plasmid DNA manufacturing processes are needed which meet...
Supercoiled state corresponds to the active form for plasmid applications. The relaxed circular for...
The interest in plasmid DNA (pDNA) as a biopharmaceutical has been increasing over the last several ...
Plasmids DNA (pDNA) are small, circular pieces of DNA used to deliver genetic information and are ke...
Renewed interest in plasmid DNA (pDNA) production is the result of its role in the supply chain of n...
Advances in recombinant DNA technology are leading to a novel generation of medicines for the treatm...