The Golgi Complex of the Hepatogyte: Isolation and Partial Enzomological Characterization

The elements of the Golgi complex of the hepatocyte are characterized by their content of 30-80m osmiophilic particles, which have been identified by others as very low density lipoprotein. The vast majority of such particles in the cell are in the elements of the Golgi complex. Moreover, the number in the complex can be increased still further by acute ethanol intoxication. This light lipoprotein content afforded the opportunity to isolate a purified fraction of Golgi-derived vesicles from the liver cell, since the content itself would make these vesicles lighter than vesicles derived from other intracellular sources (which do not contain lipoprotein particles) and since the clusters of particles could serve as a unique marker for Golgi-derived material