Production and Characterization of Immunoglobuline Yolk as Anti Antigen Membrane Toxoplasma gondii

Toxoplasma gondii is an obligate parasite intracellular which can infection human and other mammalian. Immunoglobulin Y technology offers several advantages better than antibody production in mammals. This research is aimed to get immunoglobulin Y from egg yolk, and to find the characterization of immunoglobuline Y according to molecular weight by SDS PAGE and targeted protein with antibodies using Western Blot. This research divided from many step: culture tachyzoites of T. gondii fromintraperitoneal fluid, preparation of membrane antigen tachyzoite of T. gondii, then immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blot. The result of this research showed that immunoglobulin Y from egg yolk can produced antibody against protein membrane of T. gondiiandprofile protein immunoglobuline Y according SDS PAGE has molecular weight 179,8 kDa. Immunoglobuline Y was analyze by Western Blot can recognize antigen epitope of T.gondii on molecular weight 35,7kDa and 78,8 kDa. Keywords: Toxoplasma gondii, anti membrane T.gondii, immunoglobulin Y anti membran