Antibacterial Activity of Secondary Metabolite Compounds in Ethyl Acetate Extract of Rumput Mutiara (Hedyotis corymbosa (L.) Lamk)

Abstract: Plant Rumput Mutiara (Hedyotis corymbosa (L.) Lamk) is one of the family species of Rubiaceae which is used as a traditional medicine that is effective in healing boils, acne, antiinflammatory, and anticancer. Research methods include maceration, fractionation, purification, class test and bioactivity test with Kirby-Bauer diffusion method using Escherichia coli (E.coli) bacteria. The results of this research are pure isolates with white crystal needle shape with melting point 137-138oC. Pure isolates were analyzed using FTIR, and by the IR spectrum which showed the absorption band at wave numbers 3435.22 cm-1 indicated the presence of –OH, CH3 and -CH2-aliphatic groups (2956.87 cm-1; 2935.66 cm-1; 2893.22 cm-1; and 2866.22 cm-1), C = C (1641.42cm-1),-CH3 and -CH2- bending (1462.04 cm-1 and 1377.17 cm-1), CO (1056.99 cm-1), and = CH (964.41 cm-1). Based on the result, it showed that the isolate was a steroid group which has low antibacterial activity against E.coli with inhibition power of 10 mm