Add to ORKGWe present a method for cultivation of yeast cells and analysis of secreted enzymes in nanoliter (nL) droplets. We combined droplet microfluidics with a spotting platform on top of a fluorescence microscope. Yeast cells are encapsulated in nanoliter volumes using a T-junction and transferred via capillary onto a glass plate with currently up to 10000 hydrophilic spots. Droplets on the plate are post-processed by adding further assay compounds and can be analysed by fluorescence microscopy and mass spectrometry
Yeast is the prime workhorse in the production of beer, wine, chocolate, spirits, biofuels and, more...
The ability to engineer enzymes has wide ranging applications in industry and biotechnology. Recentl...
© 2018 The Royal Society of Chemistry. We present here a new method to easily and reliably generate ...
High-throughput screening of cell-secreted proteins is essential for various biotechnological applic...
Background : Droplet-based microfluidics is becoming an increasingly attractive alternative to micro...
A high-throughput method for single cell screening by microfluidic droplet sorting is applied to a w...
Microfluidics-based sorting of cells after encapsulation in water-in-oil or water/oil/water droplet ...
Cell factories are cells that have been engineered to produce a compound of interest, ranging from b...
Microfluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be a...
Cell factory development is critically important for efficient biological production of chemicals, b...
Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at hig...
The ability to perform laboratory operations on small scales using miniaturized devices provides num...
© 2017, National Academy of Sciences. All rights reserved.Ultrahigh-throughput screening (uHTS) tech...
The ability to perform laboratory operations on small scales using miniaturized devices provides num...
International audience11 Application of droplet-based microfluidics for the screening of microbial l...
Yeast is the prime workhorse in the production of beer, wine, chocolate, spirits, biofuels and, more...
The ability to engineer enzymes has wide ranging applications in industry and biotechnology. Recentl...
© 2018 The Royal Society of Chemistry. We present here a new method to easily and reliably generate ...
High-throughput screening of cell-secreted proteins is essential for various biotechnological applic...
Background : Droplet-based microfluidics is becoming an increasingly attractive alternative to micro...
A high-throughput method for single cell screening by microfluidic droplet sorting is applied to a w...
Microfluidics-based sorting of cells after encapsulation in water-in-oil or water/oil/water droplet ...
Cell factories are cells that have been engineered to produce a compound of interest, ranging from b...
Microfluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be a...
Cell factory development is critically important for efficient biological production of chemicals, b...
Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at hig...
The ability to perform laboratory operations on small scales using miniaturized devices provides num...
© 2017, National Academy of Sciences. All rights reserved.Ultrahigh-throughput screening (uHTS) tech...
The ability to perform laboratory operations on small scales using miniaturized devices provides num...
International audience11 Application of droplet-based microfluidics for the screening of microbial l...
Yeast is the prime workhorse in the production of beer, wine, chocolate, spirits, biofuels and, more...
The ability to engineer enzymes has wide ranging applications in industry and biotechnology. Recentl...
© 2018 The Royal Society of Chemistry. We present here a new method to easily and reliably generate ...