Anthelmintics in plants - uptake, biotransformation and transcriptional response

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Mgr. Eliška SYSLOVÁ Supervisor prof. Ing. Barbora SZOTÁKOVÁ, Ph.D. Consultant RNDr. Radka PODLIPNÁ, Ph.D. Title of Doctoral Thesis: Anthelmintics in Plants - Uptake, Biotransformation and Transcriptional Response The use of anthelmintics, drugs against parasitic worms, is almost necessary in veterinary medicine today. However, anthelmintics may enter the environment with the excrements of treated animals, and influence non-target organisms there. Plants which can receive, biotransform or accumulate these substances, are an integral part of the environment. Biotransformation of anthelmintics may affect the capacity of the antioxidant system, interfere with endogenous plant metabolism or polyphenol biosynthesis where plants use the same detoxifying enzymes. The aim of this thesis is to study the influence of the most commonly used anthelmintics albendazole (ABZ), fenbendazole (FBZ), flubendazole (FLU) and ivermectin (IVM) on plants. To study their uptake by plants, their biotransformation in plants and transcriptional response of plant cells to their presence. The results of the dissertation include the finding that model systems of cell suspensions and in vitro regenerants of Plantago lanceolata..