Efficient Bacterial Producing Antibiotics in the Soil Sample

As the earth’s body, the soil is a mixture of organic matter, mineral, and liquid, which plays the role as a medium for plant growth, water storage, supply earth’s atmosphere, and a habitat for organisms. Research indicates that one gram of soil contains tens of thousands of bacterial species and billions of individual cells. Although there are many anti-bacteria cells in the human immune system, the infection caused by ESKAPE pathogens are often challenging to treat due to their antibiotic-resistant. In the experiment, our purpose is to use healthy soil to find the more efficient candidates for bacterial producing antibiotics. Collected the peaty soil sample from the backyard in Shawnee, KS and diluted the soil to find the pure potential candidates that with the ideal zones of inhibition were present. After the potential candidates were determined, created a master plate to focus on the potential candidates and check the pure. Finally, testing the candidates against gram-positive and gram-negative pathogens to identify the bacteria inhibition result. As the results, six pure candidates on the master plate were tested their producing antibiotics. We used E.coli as gram-negative tester strain and E. faecalis as gram-positive tester strain screening for antibiotics. Number 3 candidate was successfully found the clear inhibition zone on both gram-negative and gram-positive testes strains. Therefore, number 3 candidate is a new soil antibiotic found in the microbiology lab