Albumins and their processing machinery are hijacked for cyclic peptides in sunflower
- Mylne, Joshua S.
- Colgrave, Michelle L.
- Daly, Norelle
- Chanson, Aurelie H.
- Elliott, Alysha G.
- McCallum, Emily J.
- Jones, Alun
- Craik, David J.
DOIAbstract
The cyclic peptide sunflower trypsin inhibitor 1 (SFTI-1) blocks trypsin and is a promising drug lead and protein engineering scaffold. We show that SFTI-1 and the newfound SFT-L1 are buried within PawS1 and PawS2, precursors for seed storage protein albumins. Proalbumins are matured by asparaginyl endopeptidase, which we show is required to liberate both ends of SFTI-1 as well as to mature PawS1 albumin. Thus, these peptides emerge from within an albumin precursor by the action of albumin's own processing enzyme
Add to ORKG