Quantitation of paracetamol by liquid chromatography-mass spectrometry in human plasma in support of clinical trial
- Plewes, K
- Tarning, JO
- Dondorp, AM
- Ghose, A
- Wong, HT
- Kam, RK
- Chan, MH
DOIAbstract
Background: Paracetamol is a well-tolerated antipyretic widely used in severe malaria management. The study aimed to develop and validate a rapid liquid chromatography mass spectrometry (LC-MS/MS) assay to quantify paracetamol in plasma from patients with severe malaria. Results: Plasma samples were extracted with organic solvent and assayed in less than 5.5 minutes. The assay response was linear (0.125-50 mg/L) with total intra- and inter-assay imprecision of <1.4%, which were considerably lower than most published reports. Conclusions: We developed, validated and applied a rapid, and small volume LC-MS/MS assay with high precision and accuracy for plasma paracetamol quantitation in 989 samples from 62 patients with severe malaria. The s...
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