OBJECTIVE: To compare the TCR beta repertoire of peripheral blood CD8 enriched (CD8+) and depleted (CD8-) T cells in rheumatoid arthritis (RA) patients and controls using CDR3 length analysis (spectratyping). METHODS: CD8+ and CD8- T cells were separated from 14 RA patients and 12 controls, using magnetic beads coated with anti-CD8 monoclonal antibodies. cDNA was prepared as the template for amplification with 22 V beta-C beta primer pairs. The products were resolved by electrophoresis in an ABI373 sequencer using GENESCAN software. Expansions were identified as dominant CDR3 lengths, where the area underlying the corresponding peak exceeded the sum of the areas of the two adjacent peaks. This method was validated by sequencing 10 samples d...
<p>PBMC from RA patients were isolated and FACS analyses from live cells were performed (PI staining...
OBJECTIVE: To assess variation in peripheral blood B lymphocyte subsets in rheumatoid arthritis (RA)...
Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-li...
Objective—To compare the TCRâ reper-toire of peripheral blood CD8 enriched (CD8+) and depleted (CD8−...
Rheumatoid arthritis (RA) is an autoimmune disease involving the synovial membrane of peripheral joi...
Previous work suggested that expanded CD8+ T-cell clones in the synovial fluid (SF) of HLA-B27+ pati...
Polymerase chain reaction (PCR) technology was employed to examine peripheral blood and synovial T c...
Objective. CD8+ T cells are abundant in rheumatoid arthritis (RA). However, their role in disease pa...
OBJECTIVE: To profile quantitatively the T-cell repertoire in multiple joints and peripheral blood o...
OBJECTIVE: To look for evidence of T lymphocyte expansions in the blood and synovial fluid (SF) of p...
Objective CD8+T cells are abundant in rheumatoid arthritis (RA). However, their role in the disea...
Background: Multiple lymphocyte subsets like T and B cells have been connected to joint infiltration...
The pattern of T cell receptor V gene expression by lymphocytes from rheumatoid synovial fluid and p...
We have demonstrated a stable expansion of CD8+ T cells in the peripheral blood of a child with chro...
Rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA) are characterized by chronic infla...
<p>PBMC from RA patients were isolated and FACS analyses from live cells were performed (PI staining...
OBJECTIVE: To assess variation in peripheral blood B lymphocyte subsets in rheumatoid arthritis (RA)...
Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-li...
Objective—To compare the TCRâ reper-toire of peripheral blood CD8 enriched (CD8+) and depleted (CD8−...
Rheumatoid arthritis (RA) is an autoimmune disease involving the synovial membrane of peripheral joi...
Previous work suggested that expanded CD8+ T-cell clones in the synovial fluid (SF) of HLA-B27+ pati...
Polymerase chain reaction (PCR) technology was employed to examine peripheral blood and synovial T c...
Objective. CD8+ T cells are abundant in rheumatoid arthritis (RA). However, their role in disease pa...
OBJECTIVE: To profile quantitatively the T-cell repertoire in multiple joints and peripheral blood o...
OBJECTIVE: To look for evidence of T lymphocyte expansions in the blood and synovial fluid (SF) of p...
Objective CD8+T cells are abundant in rheumatoid arthritis (RA). However, their role in the disea...
Background: Multiple lymphocyte subsets like T and B cells have been connected to joint infiltration...
The pattern of T cell receptor V gene expression by lymphocytes from rheumatoid synovial fluid and p...
We have demonstrated a stable expansion of CD8+ T cells in the peripheral blood of a child with chro...
Rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA) are characterized by chronic infla...
<p>PBMC from RA patients were isolated and FACS analyses from live cells were performed (PI staining...
OBJECTIVE: To assess variation in peripheral blood B lymphocyte subsets in rheumatoid arthritis (RA)...
Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-li...