We have investigated whether active RNA polymerase I, the enzyme responsible for transcribing ribosomal RNA, is immobilized by attachment to a large subnuclear structure in HeLa cells. As unphysiological salt concentrations induce artifacts, we have used isotonic conditions throughout the preparative and analytic procedures. Cells are encapsulated in agarose microbeads and lysed in Triton and a 'physiological' buffer; then soluble proteins and RNA diffuse out through the agarose pores to leave encapsulated chromatin. This can be manipulated without aggregation but is accessible to molecular probes; it retains the replicational and transcriptional activities of the living cell. After treatment with a restriction endonuclease, most chromatin ...
Methods for isolating nuclei, nucleoli and chromatin from. Physarum polycephalum which retain high l...
RNA polymerase I (Pol I) is a highly efficient enzyme specialized in synthesizing most ribosomal RNA...
It is widely assumed that active RNA polymerases track along their templates to produce a transcript...
Nascent transcripts in permeabilized HeLa cells were elongated by approximately 30-2,000 nucleotides...
RNA polymerase (Pol) I-transcribed ribosomal genes of budding yeast exist as a tandem array (about 1...
It is now eleven years since Weiss first detected RNA polymerase activity in extracts of rat liver c...
Rabbit antibodies to RNA polymerase I from a rat hepatoma have been used to localize the enzyme in a...
In mammalian cells, active ribosomal genes produce the 18S, 5.8S and 28S RNAs of ribosomal particles...
Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are a...
Electron microscopy was used to monitor the fate of reconstituted nucleosome cores during in vitro t...
Mammalian cell nuclei contain three RNA polymerases (RNAP I, RNAP II and RNAP III), which transcribe...
Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are a...
Various complexes that contain the core subunits of RNA polymerase II associated with different tran...
We have studied the reconstitution of chromatin-like structures in vitro, using purified RNA polymer...
RNA polymerase I (Pol I) is a highly processive enzyme that transcribes ribosomal DNA (rDNA) and reg...
Methods for isolating nuclei, nucleoli and chromatin from. Physarum polycephalum which retain high l...
RNA polymerase I (Pol I) is a highly efficient enzyme specialized in synthesizing most ribosomal RNA...
It is widely assumed that active RNA polymerases track along their templates to produce a transcript...
Nascent transcripts in permeabilized HeLa cells were elongated by approximately 30-2,000 nucleotides...
RNA polymerase (Pol) I-transcribed ribosomal genes of budding yeast exist as a tandem array (about 1...
It is now eleven years since Weiss first detected RNA polymerase activity in extracts of rat liver c...
Rabbit antibodies to RNA polymerase I from a rat hepatoma have been used to localize the enzyme in a...
In mammalian cells, active ribosomal genes produce the 18S, 5.8S and 28S RNAs of ribosomal particles...
Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are a...
Electron microscopy was used to monitor the fate of reconstituted nucleosome cores during in vitro t...
Mammalian cell nuclei contain three RNA polymerases (RNAP I, RNAP II and RNAP III), which transcribe...
Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are a...
Various complexes that contain the core subunits of RNA polymerase II associated with different tran...
We have studied the reconstitution of chromatin-like structures in vitro, using purified RNA polymer...
RNA polymerase I (Pol I) is a highly processive enzyme that transcribes ribosomal DNA (rDNA) and reg...
Methods for isolating nuclei, nucleoli and chromatin from. Physarum polycephalum which retain high l...
RNA polymerase I (Pol I) is a highly efficient enzyme specialized in synthesizing most ribosomal RNA...
It is widely assumed that active RNA polymerases track along their templates to produce a transcript...