Image-based artefact removal in laser scanning microscopy

Recent developments in laser scanning microscopy have greatly extended its applicability in cancer imaging beyond the visualisation of complex biology, and opened up the possibility of quantitative analysis of inherently dynamic biological processes. However, the physics of image acquisition intrinsically means that image quality is subject to a trade-off between a number of imaging parameters including resolution, signal-to-noise ratio, and acquisition speed. We address the problem of geometric distortion, in particular jaggedness artefacts that are caused by the variable motion of the microscope laser, by using a combination of image processing techniques. Image restoration methods have already shown great potential for post-acquisition image analysis. The performance of our proposed image restoration technique was first quantitatively evaluated using phantom data with different textures, and then qualitatively assessed using in vivo biological imaging data. In both cases, the presented method, comprising a combination of image registration and filtering, is demonstrated to have substantial improvement over state-of-the-art microscopy acquisition methods