Biochemické parametry zony pellucidy prasečích oocytů během folikulogeneze

Polyspermic fertilization is one of major problems during in vitro fertilization of porcine oocytes. Zona pellucida is an extracellular matrix, that surrounds growing mammalian eggs and is responsible for polyspermy block after fertilization. Porcine zona pellucida is composed of three glycoproteins called ZP2, ZP3 and ZP4. This study is aimed to modifications of ZP3 protein of oocytes with different developmental competence. No significant differences were found in expression of ZP3 protein in oocytes with lower developmental competence (follicular size S), in compared to oocytes with higher developmental competence (follicular size M). However, it was found significant differences in acidification of ZP3 protein of immature oocytes removed from follicles of size S, in compared to oocytes removed from follicles of size M. Moreover, using Western blot analysis, it was detected band in area > 50 kDa, which could play an essential role during fertilization of oocytes