Mikroorganismy s dekarboxylázovou aktivitou z mléka a mléčných výrobků

This thesis deals with bacteria that are capable decarboxylase one or more amino acids and thus contribute to the formation of biogenic amines. The aim of this work was to characterize the bacteria with decarboxylase activitthe Escherichia coli, Bacillus, Lactobacillus, Enterococcus isolated from milk and dairy products. Microorganisms were inoculated into tubes with diagnostic medium. To test was used seven amino acids. Incubation tubes conducted at 6, 25, 30 and 37 ° C for 10 days during which the color change was monitored. The tested isolates of Escherichia coli were detected decarboxylase activity, especially for tyrosine and ornithine. The bacterium Bacillus licheniformis was found decarboxylase activity, especially on tyrosine. The bacteria Lactobacillus isolates tested were shown the strongest decarboxylase activity especially for arginine. The bacteria Enterococcus was demonstrated the strongest decarboxylase activity especially for arginine. Furthermore, the observed production of carbon dioxide and formation of biogenic amines contaminating microorganisms in cow's and goat's curd