Effect of storage media, temperature, and time on preservation fecal symples containing selcted zoonotic protist for PCR analysis

Resting stages of parasites are very well resistant to adverse effects of the external environment. They have the ability to survive for long periods and to accommodate to such effects. One of the accurate methods for detection of C. parvum and E. cuniculi is PCR. As the most appropriate storage medium for the droppings collected in the field is recommended RNA later and 2,5 % potassium dichromate. Samples fixed in this way may be stored at temperatures ranging from -20 to 21 °C, event if there is no possibility of storing them in a refrigerator