The verification of the mass induction of triploidy in brook charr in operating conditions.

This bachelor thesis deals with the induction of triploidy in brook trout, Salvelinus fontinalis under farm conditions using a heat- and /or hydrostatic pressure shock. The first part involves the issues of polyploidy in fish, its induction, detection methods of polyploidy and its contribution to aquaculture. The second part deals with the technological process of induction of triploidy and subsequently, with evaluation of the effectiveness of both types of the shock upon ploidy level determine by flow cytometry. In this experiment, heat shock induced 80% of triploids in contrary to the hydrostatic pressure shock inducing triploidy in 100%. The pressure shock induced triploidy in brook trout more effectively than the heat shock and provided better operational results. Moreover, pressure shock displayed better fertilization of eggs, higher hatchability, and also higher percentage of triploids