Response of animal cells to the extract, obtained from the colony matrix of Pectinatella magnifica

The aim of this thesis was to determine whether colonies freshwater bryozoans Pectinatella magnifica produce biologically active substances. On the basis of knowledge of the course of the growth curves of cell culture mouse fibroblasts were developed models for biological assays to determine the biological potential of substances contained in the extract of lyophilisate colonies of P. magnifica. Tests demonstrated the presence of substances with biological activity. Next part of this work was also survey the occurrence and distribution of the reference type to Vltavotýnsku, including the monitoring of basic parameters of the aquatic environment. It was explored in two years (2012 and 2013) repeated a total of 10 sites on rivers Vltava and Lužnice, the incidence was detected at 5 locations