Detection Methods for Small Analytes: Improvement of Novel SPR Technology Detection of Toxins and Toxicants

Humans are exposed to a variety of toxic substances in their day-to-day activities that may have adverse health effects if not properly identified. An integrated dual-mode instrument that combines grating-coupled surface plasmon resonance (GCSPR) and grating-coupled surface plasmon coupled emissions (GCSPCE) should allow for the efficient detection of toxic analytes in a high-content system using gold-coated superparamagnetic nanobeads. The goal of this study is to validate the binding characteristics of the reagents intrinsic to this innovative assay methodology. Competition enzyme-linked immunosorbent assays (ELISA) and surface plasmon resonance (SPR) assays were conducted on NNK toxicants and SEB toxins, which were the prototype toxicant and toxin being analyzed