Luteipulveratus halotolerans sp. nov., a novel actinobacterium (Dermacoccaceae) from Sarawak, Malaysia.

The taxonomic position of an actinobacterium strain, C296001T, isolated from a soil sample collected in Sarawak, Malaysia, was established using a polyphasic approach. Phylogenetically, strain C296001T is closely associated with the genus Luteipulveratus that forms a distinct monophyletic clade with the only described species, L. mongoliensis NBRC 105296T. The 16S rRNA gene sequence similarity between strain C296001T and L. mongoliensis is 98.7%. DNA-DNA hybridization results showed that the relatedness of the novel strain to L. mongoliensis was only 21.5%. The G+C content of strain C296001T DNA is 71.7 mol%. Using a PacBio RS II system whole genome sequences for strains C296001T and NBRC 105296T were obtained. The determined genome sizes of 4.5 Mbps and 5.4 Mbps are similar to those of other Dermacoccaceae. The cell-wall peptidoglycan containing lysine, alanine, aspartic acid, glutamic acid and serine represents the peptidoglycan type A4α L-Lys-L-Ser-D-Asp. The major menaquinones are MK-8(H4), MK-8, and MK-8(H2). Phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and phosphoglycolipid are the polar lipids, while the whole-cell sugars are galactose and fucose. The major fatty acids are iso-C16:0, anteiso-C17:0, iso-C16:1 H, anteiso-C17:1 ω9c, iso-C18:0, and C17:0 10-methyl. Phenotypic and chemotaxonomic analyses showed that C296001T had typical characteristics of members of the genus Luteipulveratus, with the main chemotaxonomical difference occurring in the whole cell sugar content. Various phenotypic differences between the two strains were observed. Based on the morphological, physiological, biochemical, and chemotaxonomic evidence, it is proposed that strain C296001T be classified as a novel species in the genus Luteipulveratus, for which the name Luteipulveratus sarawakiensis sp. nov. is recommended. The type strain is designated C296001T (“=ATCC…=JCM…”)