Background: Getting access to authentic human drug metabolites is an important issue during the drug discovery and development process. Employing recombinant microorganisms as whole-cell biocatalysts constitutes an elegant alternative to organic synthesis to produce these compounds. The present work aimed for the generation of an efficient whole-cell catalyst based on the flavin monooxygenase isoform 2 (FMO2), which is part of the human phase I metabolism. Results: We show for the first time the functional expression of human FMO2 in E. coli. Truncations of the C-terminal membrane anchor region did not result in soluble FMO2 protein, but had a significant effect on levels of recombinant protein. The FMO2 biocatalysts were employed for sub...
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set...
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set...
Flavin-containing monooxygenases (FMOs) are, after cytochromes P450, the most important monooxygenas...
Rec. h. flavine containing monooxygenases (FMOs), isoforms 1-5, were cloned and expressed in E. coli...
Deazaflavin-dependent whole-cell conversions in well-studied and industrially relevant microorganism...
Flavin-containing mono-oxygenases are known as potent drug metabolizing enzymes, providing complemen...
Although biocatalytic transformation has shown great promise in chemical synthesis, there remain sig...
FMO enzymes (FMOs) play a key role in the processes of detoxification and/or bioactivation of specif...
Introduction: Flavin-containing monooxygenases (FMOs) play an important role in drug metabolism. / A...
Flavin-containing mono-oxygenases are known as potent drug-metabolizing enzymes, providing complemen...
The flavin-containingmonooxygenase (FMO) family of enzymesoxygen-ates nucleophilic xenobiotics and e...
This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published arti...
Mammalian flavin-containing monooxygenases, which are difficult to obtain and study, play a major ro...
F-420-dependent enzymes are found in many microorganisms and can catalyze a wide range of redox reac...
Flavin-containing monooxygenases (FMOs) are emerging as effective players in oxidative drug metaboli...
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set...
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set...
Flavin-containing monooxygenases (FMOs) are, after cytochromes P450, the most important monooxygenas...
Rec. h. flavine containing monooxygenases (FMOs), isoforms 1-5, were cloned and expressed in E. coli...
Deazaflavin-dependent whole-cell conversions in well-studied and industrially relevant microorganism...
Flavin-containing mono-oxygenases are known as potent drug metabolizing enzymes, providing complemen...
Although biocatalytic transformation has shown great promise in chemical synthesis, there remain sig...
FMO enzymes (FMOs) play a key role in the processes of detoxification and/or bioactivation of specif...
Introduction: Flavin-containing monooxygenases (FMOs) play an important role in drug metabolism. / A...
Flavin-containing mono-oxygenases are known as potent drug-metabolizing enzymes, providing complemen...
The flavin-containingmonooxygenase (FMO) family of enzymesoxygen-ates nucleophilic xenobiotics and e...
This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published arti...
Mammalian flavin-containing monooxygenases, which are difficult to obtain and study, play a major ro...
F-420-dependent enzymes are found in many microorganisms and can catalyze a wide range of redox reac...
Flavin-containing monooxygenases (FMOs) are emerging as effective players in oxidative drug metaboli...
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set...
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set...
Flavin-containing monooxygenases (FMOs) are, after cytochromes P450, the most important monooxygenas...