This work deals with the issue of using liquid biofuels in transport. The first part concentrates on technical aspects of their use. It summarizes sources for producing biofuels, characterizes individual production technologies and states physical and chemical properties of their products, ways of distribution and ecological aspects of their uses. It also briefly describes the history of using biofuels in the Czech Republic. Further on, it describes a technological unit to produce FAME by the company PREOL, a.s., Lovosice. Finally, it summarizes the existing range of biofuels used in transport in the Czech Republic. The second part contains experimental measuring on the motor brake of tractor Zetor 6211 in workshops of Agricultural faculty ...