The investigation of markers in urine with a focus of IMC

Abstract Urinary passage infection is one of the most frequent diseases in both children and adult populations. The UPI (Urinary Passage Infection) diagnostics is based on clinical data obtained from the sick, i.e. on anamnestic data, clinical current problems and examination of urine and blood. When examining urine, it is possible to detect infection markers, which show evidence of an acute or chronic infection. To ensure correct interpretation, it is necessary to comply with the required methodologies in both pre-analytic and actual analytic phases. The objective of this bachelor thesis is to prove that within the studies and preparation of the bachelor thesis, I mastered the laboratory methodology of examination of UPI markers in urine. I processed the biological material both independently and under expert supervision. I worked in biochemical and microbiological laboratories, where I myself performed the examination or participated in the examinations of inflammatory markers on devices. Within the preparation of the bachelor thesis, I examined samples of urine from children as well as adults. Altogether I examined 120 samples of urine chemically, microscopically and microbiologically. During the chemical examination, I worked under the expert supervision of a laboratory worker and used the automatic spectrophotometer Clinitek Atlas. I examined osmolality with the use of an osmometer. To identify important bacteriuria, the transport set Uricult was used. The reading was performed in compliance with the enclosed manuals. I assessed the results of the examination after a consultation with a doctor, namely in the clinical-biochemical department of the hospital Nemocnice Jindřichův Hradec a.s., and interpreted them after a consultation with Prof. MUDr. Miloš Velemínský, CSc. All examinations were documented. The result of the bachelor thesis is the presentation of own results obtained by examination of urine for inflammatory markers of UPI and their documentation