Influence of water temperature on toxicity of free ammonia for fish and on amount of ammonia produced by fish

Determination of acute toxicity of free ammonia for fish in dependence on water temperature and determination of production of ammonia by fish in dependence of water temperature It was made a test of acute toxicity on akutní toxicity on Poecilia reticulata according to ČSN EN ISO 7346-2 and determination of acute lethal toxicity of substances for fish. Next was made test on excretion of ammonia by common carp in different temperatures of water. Fish were divided after a two pieces into basins.The water temperature was kept in basins on two level. Next test was realized in a similar way with a difference, that an amyloid gel with an addition of NH4Cl was aplicated by a probe to the digestive tract of fish. To control fish were aplicated the amyloid gel without an addition of NH4Cl. It was observed concetration of ammonia in water and the fish were bleeded to set haematocrit and biochemical values. On grounds of these values will be analyzed production of ammonia by one species of fish in dependence on water temperature