Příprava modifikovaných nukleosidů, nukleotidů a oligonukleotidů nesoucích komplexy kovů

  • Vrábel, Milan
Publication date
January 2008

Abstract

Series of modified nucleosides and oligonucleotides bearing metal complexes were synthetized. Palladium catalyzed aqueous-phase cross-coupling reactions were used as key step in the synthesis of modified metallo-labeled nucleosides, nucleotides and oligonucleotides. The corresponding Fc modified dNTPs were good sustrates for DNA polymerases and were efficiently incorporated to DNA by primer extension (PEX). The modified nucleic acids are applicable as tools in bioanalysis

Extracted data

We use cookies to provide a better user experience.