Using the Glucose Oxidase Peroxidase System in Enzyme Kinetics

An expeimental protocol is described for a biochemistry laboratory on enzyme kinetics using glucose oxidase coupled to a horseradish peroxidase/colored dye system to yield an easily visualized and quantitated product. The experiment is designed to illustrate the concepts of specific activity, substrate specificity and kinetic parameters. Optional extensions of the protocol could explore parameters of coupled enzyme systems, reversible inhibition and enzyme immobilization