A Filter-Based Propidium Monoazide Technique to Distinguish Live from Membrane-Compromised Microorganisms Using Quantitative PCR
- Hellein, Kristin N.
- Kennedy, Elizabeth M.
- Harwood, Valerie J.
- Gordon, Katrina V.
- Wang, Shiao Y.
- Lepo, Joe Eugene
DOIAbstract
Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. (C) 2012 Elsevier B.V. All rights reserved
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