Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa

PURPOSE. The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods. miRNAs-mRNA interaction networks were generated for analysis of biological pathways involved in retinal degeneration. RESULTS. Of more than 1900 miRNAs analyzed, we selected 19 miRNAs on the basis of (1) a significant differential expression in rd10 retinas compared with control samples and (2) an inverse expression relationship with predicted mRNA targets involved in biological pathways relevant to retinal biology and/or degeneration. Seven of the selected miRNAs have been associated with retinal dystrophies, whereas, to our knowledge, nine have not been previously linked to any disease. CONCLUSIONS. This study contributes to our understanding of the etiology and progression of retinal degeneration.Supported by the Fundacion Jesus de Gangoiti Barrera and from the Basque Government's Department of Industry and Education Grants SAIOTEK-PE11BN002, PC12BN001, and DEPLC13/002 (AA, JRE); funds from Foundation of Patients of Retinitis Pigmentosa of Gipuzkoa (Retinosis Gipuzkoa Begisare) (OB); a grant from the Fundacion Mutua Madrilena (OB); Basque Government's Department of Education grants DEDUC14/309 (MEI), Diputacion Foral de Gipuzkoa DFG15/006 (MM-C), and ELKARTEK 16/014 (MM-C); National Institute of Health Carlos III (Instituto de Salud Carlos III) Grants ISCIII: CP10/00572 (JRE), PI13/02621 (JRE); an Intensificacion Contract (ALdM) from the Basque Government's Department of Industry; and a grant from the Foundation of Patients of Retinitis Pigmentosa of Gipuzkoa (Retinosis Gipuzkoa Begisare) (JRE). JR-E is a Miguel Servet II Fellow, National Institute of Health Carlos III (Instituto de Salud Carlos III), ISCIII: CPII16/00012