Methylace histonu H3K9 v lidských krevních buňkách a v granulocytech pacientů s myeloidní leukemií

Common heterochromatin antigenic protein markers while present in human blood progenitor CD34+ cells, differentiated lymphocytes and monocytes are absent in neutrophile granulocytes and, to large extent, in eosinophiles. In acute CML and AML, strong methylation of H3K9 and all isoformes of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression and modality of treatment were observed. Reprogramming of leukemia HL60 cells to terminal differentiation by retinoic acid does not eliminate histone H3K9 methylation and the presence of HP1 isoformes from differentiated granulocytes. Our study shows for the firs time that histone H3 methylation may be dramatically changed during normal cell differentiation