UPAYA PERBANYAKAN TANAMAN PENGHASIL GAHARU (Aquilaria malaccansis Lamk) SECARA IN VITRO

Experiment to invistigate ( Plant Propagation Agarwood (Aquilaria malaccensis Lamk) by effort conservation plasma nutfah to in vitro) was done in february to november 2009 at tissue cultur laboratory of agricultur faculty, andalas university, Padang and at tissue cultur laboratory of mapeni padang. The experiment consist of tri tree stages, the first stage about give eksplan and medium for induction callus, seven (9) level consist of : 1) shootlet + medium MS; 2) Shootlet + medium WPM; 3) Shootlet + medium B5; 4) Ibu tulang daun + medium MS; 5) Ibu tulang daun + medium WPM; 6) Ibu tulang daun + media B5; 7) Petiole + medium MS; 8)Petiole + medium WPM; 9) Petiole + medium B5. The second stage about give combination concentration 2,4-D and BAP for regenaration callus, seven (9) level consist of : 1) 0,00 ppm 2,4-D + 0,00 BAP; 2) 2,50 ppm 2,4-D+ 0,00 ppm BAP; 3) 2,50 ppm 2,4-D + 1,50 ppm BAP; 4) 2,50 ppm 2,4-D + 3,00 ppm BAP (K3); 5) 5,00 ppm 2,4-D + 0,00 ppm BAP (K4); 6) 5,00 ppm 2,4-D + 1,50 ppm BAP; 7) 5,00 ppm 2,4-D + 3,00 ppm BAP; 8) 7,50 ppm 2,4-D + 0,00 ppm BAP dan 9) 7,50 ppm 2,4-D + 1,50 ppm BAP. Eksplant ibu tulang daun and medium MS were superior result than any another treatment the support growth to get explant life, callus percentage callus, callus formation, less browning for first stage. Combination concentration 2,50 ppm 2,4-D + 3,00 ppm BAP were superior result than any another treatment the support growth to get explant life, percentage regenaeration callus formatted shootlet for secallus formation, for second stage. Experiment third stage can not observated because Eahrthquake. Key words : explant, induction callus, regeneration callus, callus, shootlet, 2,4-D, auxin, NAA, Cytokinin BAP, kinetin and in vitr