Optimization of operating conditions of rotary drum bioreactor for lignin peroxidise production by solid state

Solid state bioconversion of empty fruit bunches (EFB) for lignin peroxidase (LiP) by Phanerocheate chrysosporium through optimum operating conditions of a rotary drum bioreactor (RDB) was investigated. The study was carried out in the 50L of RDB fabricated locally for 6 days of treatment. The process conditions such as initial pH of 5.5, inoculum of 5% and moisture content of 60% obtained from previous study were applied for this study. The operating conditions i.e. aeration and agitation rate for the rotary drum bioreactor were optimized by using fractional factorial design. A regression model was developed using experimental data with the consideration of linear, quadratic and interactions effects. The maximum production of LiP was 475.8 U/L while the theoretical yield was 439.6 with the optimum aeration and agitation of 30 l/min and 15 rpm respectively using RDB by solid state bioconversion