Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]

To determine the manganese requirement necessary for optimal development of lesser centaury [Centaurium pulchellum (Sw.) Druce] in vitro, we investigated the effect of exogenously applied Mn on different developmental processes such as growth, flowering, fruiting, and seed germination. The application of Mn had no effect on stem length, except at the highest concentration of 10-2 M, which was inhibitory. In addition, C. pulchellum plants were capable of in vitro flowering and fruiting even on media without added Mn. However, Mn content in the media affected seed dimensions, since both length and width of the seeds increased with increasing Mn concentration. Moreover, both excess and absence of Mn in the media caused appearance of necrotic plants. Exogenously applied Mn had no effect on seed germination percentage, except at concentrations greater than 3x10-3 M.U ovom radu proučavani su efekti egzogeno apliciranog mangana tokom procesa rasta, cvetanja, plodonošenja i klijanja semena kičice sitne [Centaurium pulchellum (Sw.) Druce], gajene u kulturi in vitro. Rezultati su pokazali da Mn nema efekta na dužinu stabla. Jedino je najviša primenjena koncentracija Mn od 10-2 M imala inhibitorni efekt na posmatrani proces. Biljke kičice sitne mogu ne samo da rastu, već i da cvetaju i plodonose na medijumu bez apliciranog Mn. Međutim, dimenzije semena u mnogome zavise od koncentracije Mn u podlozi. Sa povećanjem koncentracije Mn u podlozi, raste i širina i dužina semena. Izostanak Mn u podlozi, kao i njegove visoke koncentracije uzrokovale su pojavu nekrotičnih biljaka. Koncentracije Mn niže od 3 x 10-3 M nisu imale efekta na procenat proklijalih semena.Projekat ministarstva br. 14303