Superoxide dismutase (SOD) and catalase (CAT) activities in some tissues of three freshwater crayfish species

In our experiments, we studied superoxide dismutase (SOD) and catalase (CAT) activities in the hepatopancreas, the gills and the abdominal muscle of Noble crayfish (Astacus astacus), Stone crayfish (Austropotamobius torrentium) and Spynicheek crayfish (Orconectes limosus) from the South Morava River, Krajkovačka River and Serbian part of the Danube respectively. The specific SOD activity in the hepatopancreas of the A. astacus was significantly higher in respect to other two species (p<0.005) as well as in the hepatopancreas of A. torrentium then in O. limosus (p<0.02). The same trend was obtained for the total SOD activity in the hepatopancreas, which was significantly decreased in O. limosus compared to two other investigated species (p<0.005). The specific CAT activity in the muscle of the Austropotamobius torrentium was significantly higher in respect to Astacus astacus (p<0.005) and Orconectes limosus (p<0.005). Specific CAT activity was considerably higher in the Astacus astacus then in Orconectes limosus (p<0.02). The same trend was detected in the case of the total CAT activity in the muscle but, total CAT activity was significantly decrease in Orconectes limosus, compare to Astacus astacus (p<0.01). The obtained results show strong tissue and species specificities, indicating different metabolic activities and various environmental influences.U našim eksperimentima određivali smo aktivnost superoksid-dismutaze (SOD) i katalaze (CAT) u hepatopankreasu, škrgama i abdominalnom mišiću rečnog raka (Astacus astacus L) sa lokaliteta Južna Morava, raka kamenjara (Austropotamobius torrentium Schrank) sa lokaliteta Krajkovačka reka i američkog raka (Orconectes limosus Raf) sa lokaliteta Dunav. Specifična aktivnost SOD u hepatopankreasu rečnog raka bila je značajno veća u hepatopankreasu u poređenju sa druge dve vrste (p<0.005), kao i u hepatopankreasu Austropotamobius torrentium u odnosu na Orconectes limosus (p<0.02). Isti trend je zabeležen za ukupnu SOD aktivnost u hepatopankreasu Orconectes limosus koja je bila značajno manja u odnosu na druge dve ispitivane vrste (p<0.005). Specifična aktivnost CAT u mišiću Austropotamobius torrentium bila je značajno veća nego kod vrsta Astacus astacus i Orconectes limosus (p<0.005). Specifična aktivnost CAT bila je značajno veća kod Astacus astacus u odnosu na Orconectes limosus (p<0.02). Isti trend je zabeležen u slučaju ukupne aktivnosti CAT u mišiću, pri čemu je ukupna aktivnosti CAT bila značajno manja kod Orconectes limosus u poređenju sa Astacus astacus (p