Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages

Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SR inhibited pro-inflammatory cytokines, IL-1 beta, IL-6, and TNF-alpha production. To elucidate the mechanism by which SR inhibits NO production and iNOS expression, we investigated that SR suppressed I kappa B phosphorylation, which leads to the activation of NF-kappa B followed by degradation of I kappa B. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.This work was supported by grants from the Korea Research Institute of Standards and Science (KRISS) (2007). This work was supported by the Pioneer Research Program and the Bio & Medical Technology Development Program, through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (No. 20120001020 and 2012050100).OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000002410/14SEQ:14PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000002410ADJUST_YN:YEMP_ID:A002014DEPT_CD:458CITE_RATE:1.059FILENAME:14. (2013.09) anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages.pdfDEPT_NM:화학생물공학부EMAIL:thpark@snu.ac.krSCOPUS_YN:YCONFIRM: