Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.Copyright (2012) This article is distributed under a Creative Commons Attribution 3.0 Unported LicenseOAIID:oai:osos.snu.ac.kr:snu2012-01/102/0000027849/1SEQ:1PERF_CD:SNU2012-01EVAL_ITEM_CD:102USER_ID:0000027849ADJUST_YN:YEMP_ID:A075905DEPT_CD:718CITE_RATE:2.386FILENAME:molecules-17-02474.pdfDEPT_NM:화학교육과EMAIL:jeongdh@snu.ac.krSCOPUS_YN:YCONFIRM: