Tissue Expression of Manganese Superoxide Dismutase Is a Candidate Prognostic Marker for Glioblastoma

Background: Characterization of a rare subgroup of glioblastoma patients who survive for more than 3 years (long-term survival glioblastoma, LTSGBL, patients) may be helpful to identify prognostic factors. Materials and Methods: A molecular-profiling proteomic approach using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify prognostic factors associated with glioblastoma by comparing frozen tumor tissue from LTSGBL patients with matched samples from short-term survival glioblastoma (STSGBL) patients. Western blot (WB) analysis, reverse-transcriptase polymerase chain reaction (RT-PCR) and immmunohistochemical (IHC) staining were used for confirmation. Results: Among most candidate spots identified by 2-DE, lack of overexpression of manganese superoxide dismutase (MnSOD) in LTSGBL samples was consistently observed using WB and RT-PCR. Conclusion: These results suggest that MnSOD expression level in tumor tissue is a candidate marker for the prognosis of glioblastoma patients. Copyright (C) 2009 S. Karger AG, BaselPark CK, 2008, J CANCER RES CLIN, V134, P255, DOI 10.1007/s00432-007-0282-1Krex D, 2007, BRAIN, V130, P2596, DOI 10.1093/brain/awm204Senger D, 2003, CANCER J, V9, P214Burton EC, 2002, CLIN CANCER RES, V8, P180Kraus JA, 2001, J NEURO-ONCOL, V52, P263Ria F, 2001, BRIT J CANCER, V84, P529Shatrov VA, 2000, INT J CANCER, V85, P93Lam EWN, 1999, FREE RADICAL BIO MED, V27, P572Zhong WX, 1997, ONCOGENE, V14, P481FREEMAN BA, 1982, LAB INVEST, V47, P412WEISIGER RA, 1973, J BIOL CHEM, V248, P3582