Differential protein expression in Spirometra erinacei according to its development in its final host

This study was undertaken to identify genes involved in the growth and development of Spirometra erinacei larvae, an intestinal tapeworm of cats and dogs, within the final host. The differential protein expression at three different stages of S. erinacei, the plerocercoid larvae, 8-day-old juveniles, and adults, was compared using two-dimensional electrophoresis. Specifically or highly expressed proteins in juvenile worms were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MS)/MS. The proteome map of larvae showed fewer protein spots than juveniles or adults, whereas juveniles or adults revealed a similar protein expression profile. Eight juvenile-specific and five upregulated proteins of juveniles were identified and matched to proteins of known biological functions. These were grouped into several categories of functionally related proteins: DNA/RNA metabolism, cell trafficking, cytoskeleton, protein processing and degradation, energy metabolism, and oxidative stress. Our results give an overview of the growth and development mechanisms of cestodes within the final host and extend our understanding of parasite biology in the host-parasite relationship.Chung YB, 2008, KOREAN J PARASITOL, V46, P183, DOI 10.3347/kjp.2008.46.3.183Fielenbach N, 2008, GENE DEV, V22, P2149, DOI 10.1101/gad.1701508Ding XF, 2008, BRAIN RES, V1224, P20, DOI 10.1016/j.brainres.2008.05.051Wang YH, 2007, EXP BIOL MED, V232, P1152, DOI 10.3181/0701-RM-8Redowicz MJ, 2007, EUR J CELL BIOL, V86, P549, DOI 10.1016/j.ejcb.2007.05.007Suraweera A, 2007, J CELL BIOL, V177, P969Ji QM, 2007, EXP CELL RES, V313, P1460, DOI 10.1016/j.yexcr.2007.01.003Damer CK, 2007, EUKARYOT CELL, V6, P430, DOI 10.1128/EC.00322-06Jolly ER, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-4-r65Wen X, 2005, CELL MOL LIFE SCI, V62, P1038, DOI 10.1007/s00018-005-4547-zPARK YK, 2005, KOREAN J PARASITOL, V43, P119Westermann S, 2003, NAT REV MOL CELL BIO, V4, P938, DOI 10.1038/nrm1260Massey HC, 2003, INT J PARASITOL, V33, P1537, DOI 10.1016/S0020-7519(03)00205-4Bente M, 2003, PROTEOMICS, V3, P1811, DOI 10.1002/pmic.200300462Chemale G, 2003, PROTEOMICS, V3, P1633, DOI 10.1002/pmic.200300487Fujita N, 2003, CELL, V113, P207Weber JM, 2003, ANTIVIR RES, V58, P167, DOI 10.1016/S0166-3542(02)00212-7El Fakhry Y, 2002, PROTEOMICS, V2, P1007, DOI 10.1002/1615-9861(200208)2:83.0.CO2-GNishisaka M, 2001, INT J PARASITOL, V31, P1673, DOI 10.1016/S0020-7519(01)00278-8Laity JH, 2001, CURR OPIN STRUC BIOL, V11, P39Gothel SF, 1999, CELL MOL LIFE SCI, V55, P423Millevoi S, 1998, J MOL BIOL, V282, P111Ursic D, 1997, NUCLEIC ACIDS RES, V25, P4778KUBOTA H, 1995, EUR J BIOCHEM, V230, P3MONCMAN CL, 1995, CELL MOTIL CYTOSKEL, V32, P205LEE SH, 1990, KOREAN J PARASITOLOG, V28, P161BROWNER MF, 1989, J BIOL CHEM, V264, P12680ADROHER FJ, 1988, ARCH BIOCHEM BIOPHYS, V267, P252MARKELL EK, 1986, MED PARASITOLOGY, P5TKACHUCK RD, 1977, J PARASITOL, V63, P769