Effect of Perphenazine Hydrochloride on the Prolactin Cells of Rat Pituitary Glands

The effect of perphenazine hydrochloride on the prolactin cells of rat pituitary glands and mammary glands were studied. Virgin rats which had been primed with 0.2ml of estradiol for 10 days or not primed were injected with perphenazine intraperitoneally for 1 day or repeatedly every 5 days for 15 days. Sections of pituitary glands from them were carried out to stain the prolactin cells immunohistochemically using the specific anti-prolactin and antiRGG labeled with peroxidase. Sections of mammary glands were stained with hematoxylin and eosin . Cell counts were made from 10 representative section of corresponding levels of each gland. and the following results were obtained. Even in lower dose of perphenazine. 3mg/day for 1 day. releasing effect of prolactin granules were apparent. Perphenazine produced a significant decrease in prolactin cell counts and in the intensities of staining reaction which were recovered 20 days after injection to almost same levels of control group. The higher doses of perphenazine also developed the proliferation of lobulo-alveolar structure of the mammary glands. In estradiol-primed rats either lower or higher doses of perphenazine could revealed the same findings in both of pituitary and mammary glands. There were significant increase in adrenal weight and decrease in ovarian weight