Expression of Mx protein and interferon-alpha in pigs experimentally infected with swine influenza virus

Expression of Mx protein and interferon-a (IFN-a) was examined by immunohistochemistry in pigs experimentally infected with swine influenza virus. In infected pigs euthanatized at 1 day postinoculation (dpi), the lumen of bronchioles were filled with large numbers of mononuclear cells, small numbers of neutrophils, sloughing epithelial cells, and proteinaceous fluid. Lesions at 3 and 5 dpi were similar but less severe. Alveolar spaces were filled with neutrophils. By 7 and 10 dpi, microscopic lesions were resolved. The immunohistochemical signals for Mx protein and IFN-a antigen were confined to cells in areas that had hybridization signal for swine influenza virus. In situ hybridization and immunohistochemistry of serial sections of lung indicated that areas containing numerous swine influenza virus RNA-positive cells also have numerous Mx and IFN-a antigen-positive cells. Mean immunohistochemical scores for Mx protein-positive cells were correlated with mean immunohistochemical scores for IFN-a antigen-positive cells (rs 5 0.8799, P , 0.05). These results indicated that Mx protein and IFN-a antigen were expressed in the lung from pigs experimentally infected with swine influenza virus, but their biological functions remain to be examined.The research reported here was supported by contract research funds of the Research Institute for Veterinary Science (RIVS) from the College of Veterinary Medicine, Seoul National University, and Brain Korea 21 Project, Republic of Korea