Effect of pulse duration and pulse repetition frequency of cavitation histotripsy on erosion at the surface of soft material

Cavitation histotripsy with the short pulse duration (PD) but high pulse repetition frequency (PRF) disintegrates the tissue at a fluid interface. However, longer PD and lower PRF are used in the other focused ultrasound applications, where the acoustic radiation force, streaming, and cavitation are different, and their effects on erosion are unknown. In this study, the erosion at the surface of phantom/ex vivo tissue and the characteristics of induced bubble cloud captured by high-speed photography, passive cavitation detection, and light transmission during histotripsy exposure at varied PDs and PRFs but the same duty cycle were compared. The peak negative pressure of 6.6 MPa at the PD of 20 ms and PRF of 1 Hz began to erode the phantom, which becomes more significant with the increase of peak negative pressure, PD, and interval time between bursts. The increase of the PRF from 1 Hz to 1000 Hz, while the decrease of the PD from 20 ms to 20 μs (duty cycle of 2%) at the same energy was delivered to the gel phantom immersed in the degassed water led to the decrease of erosion volume but a slight increase of the erosion area and smoother surface. Low PRF and long PD produce the significant tissue deformation, acoustic wave refocusing, confinement of bubbles in a conical region, and more bubble dissolution after the collapse for the high acoustic scattering and light transmission signals. In comparison, high PRF and low PD produce a wide distribution of bubbles with only little wave refocusing at the beginning of cavitation histotripsy and high inertial cavitation. Acoustic emission dose has a good correlation with the erosion volume. The erosion on the porcine kidney at the varied PRFs and PDs with the same energy output showed similar trends as those in the phantom but at a slow rate. In summary, the PRF and PD are important parameters for the cavitation histotripsy-induced erosion at the interface of fluid and soft material, and they should be optimized for the best outcome.NRF (Natl Research Foundation, S’pore)Accepted versio