Structural, Mechanical And Invitro Study On Pulsed Laser Deposition Of Hydroxyapatite On Additive Manufactured Substrate

The convergence of engineering and life science has evolved into a new paradigm called Bio Additive Manufacturing (BAM). This BAM encompasses the biofabrication using cells, biological or biomaterials as building blocks to fabricate biological and bio-application oriented substance, device and therapeutic products through a broad range of engineering and biological processes. Furthermore bioactive coating on BAM surface facilitates biological fixation between the prosthesis and the hard tissue which increases the long term stability and integrity of the implant. In this paper, Hydroxyapatite (HA) Powder was coated over Additive Manufactured polyamide substrate using Pulsed Laser Deposition. Coating Morphology was characterized using SEM analysis and observed that the coating was dominated by the presence of particle droplet with different size. Compounds like tricalcium phosphate and few amorphous calcium phosphate were found along with HA which was confirmed by XRD analysis. Nano-indentation and pull-out test reveals that the layer was strong enough and withstands higher load before it peels-off. Cell line study has been carried out with MG-63 Osteosarcoma cells and results showed that the cells on coated surface multiplied better than on uncoated surface. It was evident from the results, that this kind of coating favors the long-term stability of implant fabricated using Additive Manufacturing.Published versio