Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification
- Teoh, Boon-Teong
- Sam, Sing-Sin
- Tan, Kim-Kee
- Danlami, Mohammed Bashar
- Shu, Meng-Hooi
- Johari, Jefree
- Hooi, Poh-Sim
- Brooks, David
- Piepenburg, Olaf
- Nentwich, Oliver
- Wilder-Smith, Annelies
- Franco, Leticia
- Tenorio, Antonio
- AbuBakar, Sazaly
DOIAbstract
A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without cross-reacting with other arboviruses. We assessed the diagnostic performance of the RT-RPA assay for the detection of DENV RNA in 203 serum samples of patients with clinically suspected dengue. The sera were simultaneously tested for DENV using a reverse transcripti...
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