Influence of nutrition media and essential oils on the development of Botrytis cinerea

Razvoj sive plijesni uzrokovane gljivom Botrytis cinera na jagodi praćen je tijekom tri dana na temperaturama 5˚C, 15˚C i 25˚C te je utvrđeno da je razvoj bolesti najbrži na 25˚C. Na toj temperaturi su prve promjene na plodu jagode uočene već 24 sata od inokulacije dok je nakon tri dana plod bio potpuno zahvaćem bolešću. Utjecaj 11 vrsta eteričnih ulja na razvoj B. cinerea praćen je na temperaturama 20 i 25˚C. Najbolje antifungalno djelovanje na istraživanu gljivu na obje temperature imala su ulja timijana i kima. Praćenjem utjecaja hranjivih podloga (PDA, Čapek agar, podloga od mrkve, podloga od rajčice) na rast gljive utvrdili smo da je najbolji razvoj micelija utvrđen na PDA podlozi i podlozi od rajčice.Development of grey mould caused by Botrytis cinera on strawberry was followed during three day period on temperatures 5˚C, 15˚C and 25˚C, and it was determined that the highest disease development was on 25˚C. On that temperature first changes on the strawberry fruit were noticed 24 hours after inoculation, and after 3 days fruit was completely covered with disease. Influence of 11 essential oils on development of B. cinerea was followed on temperatures 20 i 25˚C. The best antifungal activity on both temperatures had oils of thyme and cumin. Investigating the influence of media (PDA, Čapek, carrot agar and tomato agar) on fungal development it was determined that the best mycelial development is on PDA and tomato agar