Morphological characterization of pathogenic fungus Botrytis cinerea and effect of boscalide on growth of isolates in Vineyards of Srijem and Erdut

Vinova loza kao gospodarski važna kultura, danas je izložena mnogim čimbenicima koji utječu na njezinu produktivnost. Podložna je bolestima koje narušavaju kvalitetu uroda te smanjuju prinos, jedna od njih je Botrytis cinerea uzročnik sive plijesani. Gljiva parazitira skoro sve dijelove biljke naročito u uvjetima povećane vlage. Danas postoji velika zabrinutost zbog prekomjernog kemijskog suzbijanja jer je Botrytis cinerea poznata po brzom razvoju rezistentnosti. Posljedica pojave rezistentnosti dovodi do pada učinkovitosti sredstava za zaštitu bilja, što može dugoročno otežati zaštitu od biljnih bolesti na nekom području zbog nepostojanja učinkovitog sredstva. Cilj ovog istraživanja bio je utvrditi učinak Boskalida na izolate B.cinerea sa područja Erduta i Srijema. Boskalid je relativno novi fungicid koji inhibira klijanje spora. Promatranjem porasta micelija utvrđene su značajne razlike, kako između različitih izolata, tako i u različitim koncentracijama fungicida. Rezultati su pokazali jače antifugalno djelovanje pri većoj koncentraciji što može dovesti do dodatnog zagađenja okoliša. Stoga su nova saznanja ključna za uspostavu nove održive strategije zaštite vinograda koje omogućuju učinkovitiju kontrolu i smanjenu uporabu fungicida.Grapevine, as an economically important crop, today is exposed to many diseases that affects on the productivity. It can easily become infected and that affects on the quality of the crop and reduces yield, one of the diseases is the botrytis cinerea, causative agent of gray mold. The fungus parasites almost all parts of the plant, especially in conditions of increased moisture. Today, there is big concern about excessive chemical suppression because Botrytis cinerea is known for its rapid development of resistance. The consequence of resistance leads to reduction in the effectiveness of plant protection products, which can make it difficult in the long run to protect plants against diseases in some areas because of the lack of effective agent. The aim of this study was to determine the effect of Boskalid on B.cinerea isolates from the Erdut and Srijem areas. Boxalide is a relatively new fungicide that inhibits spore germination. By observing mycelial growth, significant differences were found, both between different isolates and at different concentrations of fungicides. The results showed stronger antifugal activity at higher concentrations which can lead to additional environmental pollution. Consequently, new knowledge is the key to establishing a new sustainable vineyard conservation strategy that allows for more effective control and reduced use of fungicides