Protein–polymer conjugation is a widely used technique to develop protein therapeutics with improved pharmacokinetic properties as prolonged half-life, higher stability, water solubility, lower immunogenicity, and antigenicity. Combining biochemical methods, small angle scattering (SAXS/SANS), and neutron spin–echo spectroscopy, here we examine the impact of PEGylation (i.e., the covalent conjugation with poly(ethylene glycol) or PEG) on structure and internal domain dynamics of phosphoglycerate kinase (PGK) to elucidate the reason for reduced activity that is connected to PEGylation. PGK is a protein with a hinge motion between the two main domains that is directly related to function. We find that secondary structure and ligand access to ...
PEGylation, the covalent modification of proteins with polyethylene glycol, is an abundantly used te...
We studied the effect of PEGylation on protein hydrodynamic behavior using hen egg-white lysozyme (H...
Polyethylene glycol (PEG) is a much researched polymer for the covalent modification of biological m...
Protein–polymer conjugation is a widely used technique to develop protein therapeutics with improved...
Protein–polymer conjugation is a widely used technique to develop protein therapeutics with improved...
Therapeutic proteins have increasingly been used in modern medical applications, but their effective...
<div><p>PEGylation is a successful approach to improve potency of a therapeutic protein. The improve...
PEGylation is the most widely used half-life extension strategy for protein therapeutics. While it i...
PEGylation, or addition of poly(ethylene glycol) chains to proteins, is widely used to improve deli...
PEGylation is a successful approach to improve potency of a therapeutic protein. The improved therap...
PEGylated proteins play an increasingly important role in pharmaceutical drug delivery. We recently ...
PEGylation of protein side chains has been used for more than 30 years to enhance the pharmacokineti...
Linear PEGylation, the covalent attachment of a linear polyethylene glycol polymer, is known to incr...
Site-specific PEGylation is an important strategy for enhancing the pharmacokinetic properties of pr...
The cellular interior is a crowded and dynamic environment where macromolecules occupy 20% to 30% of...
PEGylation, the covalent modification of proteins with polyethylene glycol, is an abundantly used te...
We studied the effect of PEGylation on protein hydrodynamic behavior using hen egg-white lysozyme (H...
Polyethylene glycol (PEG) is a much researched polymer for the covalent modification of biological m...
Protein–polymer conjugation is a widely used technique to develop protein therapeutics with improved...
Protein–polymer conjugation is a widely used technique to develop protein therapeutics with improved...
Therapeutic proteins have increasingly been used in modern medical applications, but their effective...
<div><p>PEGylation is a successful approach to improve potency of a therapeutic protein. The improve...
PEGylation is the most widely used half-life extension strategy for protein therapeutics. While it i...
PEGylation, or addition of poly(ethylene glycol) chains to proteins, is widely used to improve deli...
PEGylation is a successful approach to improve potency of a therapeutic protein. The improved therap...
PEGylated proteins play an increasingly important role in pharmaceutical drug delivery. We recently ...
PEGylation of protein side chains has been used for more than 30 years to enhance the pharmacokineti...
Linear PEGylation, the covalent attachment of a linear polyethylene glycol polymer, is known to incr...
Site-specific PEGylation is an important strategy for enhancing the pharmacokinetic properties of pr...
The cellular interior is a crowded and dynamic environment where macromolecules occupy 20% to 30% of...
PEGylation, the covalent modification of proteins with polyethylene glycol, is an abundantly used te...
We studied the effect of PEGylation on protein hydrodynamic behavior using hen egg-white lysozyme (H...
Polyethylene glycol (PEG) is a much researched polymer for the covalent modification of biological m...