Characterization of recombinant antibodies for detection of TNT and its derivatives

Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies