Isolasi, identifikasi dan karakterisasi vibrio spp. Patogen peislyebab vibriosis pada kerapu di balai budidaya air payau Situbondo

Abstract This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio sop,, by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 10 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibnosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnish, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on tins (pinnae pectorales. pinnae abdominaies, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly. Key words: humpback grouper (Cromileptes altivelis), Koch postulate, pathogenic Vibrio, vibriosi