Quick detection of citrus greening by PCR method with specific and universal primers

Huanglungbin or Greening is caused by ploem restricted unculturable bacterium, identified as Liberobacter. In general, Liberobacter found in Indonesia is Liberobacter asiaticum. The aim of this research was to develop the effective primers for Polymerase Chain Reaction (PCR) method in order to detect citrus greening quickly. Primers used in this research were specific Primers (011, 012c, 0A1) and universal Primers (fDl/rPI). The specific Primers (011, 012c) were more effective and specific in detecting citrus greening than those of universal Primers (fDlirP1). In addition, the advantage of specific primers was that it could be used to amplify DNA greening of 1160 by only, but could not be used to amplify DNA of other sistemic pathogens. Keywords: Huanglungbin (HLB) â liberobacter â polymerase chain reaction â quick detection â specific and universal primer