Optimasi mikrobiologik tandan kosong kelapa sawit untuk produksixilosa oleh Aspergillus sp. TnP 32=Optimiza tion of the Microbiologica I Fermentation front the Oil Pa Im Empty Fruit Bunches for ...

ABSTRACT The xylose production of the microbiological fermentation was studied from the oil palm empty fruit bunches by Aspergillus sp. The growing of TnP32 in medium containing different nitrogen concentration was studied. The effect a number of environmental parameters (e.g. temperature, pH and time incubation) was also investigated. The concentration of reducing sugar and xylose production were found to vary between different nitrogen concentration. The highest concentration of reducing sugar and xylose production of each experimental were obtained by incubating for 48 h, and after that consisted. The optimum pH for the concentration of reducing sugar and xylose production occurred in the pH range of 5.5 to 6.55.0 to 6.4 respectively. The optimum temperature for the concentration of reducing sugar and xylose production occurred at same temperature range 32 to 38°C. The research result were calculated by RSM showed that optimum concentration of reducing sugar was achieved at fermentation temperature 34.9°C, pH 5.6, nitrogen concentration 0.9%, for 48.3 h with concentration of reducing sugar 0.679 mg/mL. Xylose production at 35°C, pH 5.5, nitrogen concentration 1 % for 47.8 h, with xilose production 0,455 mg/mL Key words : oil palm EFB, microbiologic fermentation, Aspergillus sp. TnP 32, optimization processe