Pemanfaatan bekatul untuk meningkatkan produksi eritromisin dari biakan Saccharopolyspora erythraea ATCC 11635 = The Utilization of Rice Bran to Increase the Production of Erythromycin by Saccharopolyspora erythraea ATCC

The research on the utilization of rice bran to increase the production of erythromycin by Saccharopolyspora erythraea ATCC 11635, had been conducted. The fermentation of Sac. erythraea ATCC 11635 for erythromycin production was done under laboratory scale using media with and without rice bran addition. Rice bran was added with in two forms, the powder and the extract. Two different extracts of rice bran were made using aquadest at room and 40-500C temperatures. This research was conducted in three phases. The first phase was carrying out fermentations using media with 0, 20, 25, 30, 35 and 40 % additional rice bran powder. The second phase conducty fermentation 1 and 5% additional rice bran powder and extracts. The third phase was done to determine the optimum concentration from the 1-5% additional rice bran extract. As the comparison, the fermentation of Sac. erythraea ATCC 11635 in 1% TSB was also conducted. During the fermentation processes, monitoring was done on the control and each treatment cultures by determining (a) the dry cells weight of the microbe, (b) bioactivitys of erythromycin against Micrococcus luteus ATCC 9341. The bioactivitys were based on the production of erythromycin in each milligram dry cells weight microbe. The main components of the rice bran extract were determined, i. e. sucrose and glucose (Luff Schoorl method), protein (Kjeldahl method), amino acid (HPLC method) and protein molecular weight of the rice bran (Electroforesis method). The result were compared with those from TSB 1% media. It was showed that the growth occured in media containing 20-40% rice bran with range time from 30-37 days. The additional rice bran powder into the media increased the growth of Sac. erythraea ATCC 11635, while rice bran extract increase not only the growth, but also the production of erythromycin. 3%, room temperature-rice bran extract was the optimum media for erythromycin production and could be increased to 780% than control and 260% than TSB. It can be concluded that rice bran extract could be used as the production media of erythromycin product for Sac. erythraea ATCC 11635. Key Words : rice bran, production of erythromycin, Saccharopolyspora erythraea ATCC 11635